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GP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GP2 antibodies →
$725.00each
Catalog Number: LS-G118557-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Glycoprotein 2(GP2) (NM_001502), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
gp2 | ZAP75
Origin Species
Human
NCBI GenBank
NM_001502
SwissProt
P55259

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MPHLMERMVG SGLLWLALVS CILTQASAVQ RGYGNPIEAS SYGLDLDCGA PGTPEAHVCF
61DPCQNYTLLD EPFRSTENSA GSQGCDKNMS GWYRFVGEGG VRMSETCVQV HRCQTDAPMW
121LNGTHPALGD GITNHTACAH WSGNCCFWKT EVLVKACPGG YHVYRLEGTP WCNLRYCTDP
181STVEDKCEKA CRPEEECLAL NSTWGCFCRQ DLNSSDVHSL QPQLDCGPRE IKVKVDKCLL
241GGLGLGEEVI AYLRDPNCSS ILQTEERNWV SVTSPVQASA CRNILERNQT HAIYKNTLSL
301VNDFIIRDTI LNINFQCAYP LDMKVSLQAA LQPIVSSLNV SVDGNGEFIV RMALFQDQNY
361TNPYEGDAVE LSVESVLYVG AILEQGDTSR FNLVLRNCYA TPTEDKADLV KYFIIRNSCS
421NQRDSTIHVE ENGQSSESRF SVQMFMFAGH YDLVFLHCEI HLCDSLNEQC QPSCSRSQVR
481SEVPAIDLAR VLDLGPITRR GAQSPGVMNG TPSTAGFLVA WPMVLLTVLL AWLF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

534 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.