Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GP1BA / CD42b Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G123354-100
Catalog Number: LS-G123354
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD42b(GP1BA) (NM_000173), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Antigen CD42b-alpha | CD42b antigen | BDPLT3 | CD42b-alpha | DBPLT3 | Glycoprotein Ibalpha | GPIbA | GP-Ib alpha | gp1B | GPIb-alpha | BDPLT1 | BSS | CD42B | gp1BA | VWDP
- Origin Species
- Human
- NCBI GenBank
- NM_000173
- SwissProt
- P07359
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPLLLLLLLL PSPLHPHPIC EVSKVASHLE VNCDKRNLTA LPPDLPKDTT ILHLSENLLY61TFSLATLMPY TRLTQLNLDR CELTKLQVDG TLPVLGTLDL SHNQLQSLPL LGQTLPALTV121LDVSFNRLTS LPLGALRGLG ELQELYLKGN ELKTLPPGLL TPTPKLEKLS LANNNLTELP181AGLLNGLENL DTLLLQENSL YTIPKGFFGS HLLPFAFLHG NPWLCNCEIL YFRRWLQDNA241ENVYVWKQGV DVKAMTSNVA SVQCDNSDKF PVYKYPGKGC PTLGDEGDTD LYDYYPEEDT301EGDKVRATRT VVKFPTKAHT TPWGLFYSWS TASLDSQMPS SLHPTQESTK EQTTFPPRWT361PNFTLHMESI TFSKTPKSTT EPTPSPTTSE PVPEPAPNMT TLEPTPSPTT PEPTSEPAPS421PTTPEPTPIP TIATSPTILV SATSLITPKS TFLTTTKPVS LLESTKKTIP ELDQPPKLRG481VLQGHLESSR NDPFLHPDFC CLLPLGFYVL GLFWLLFASV VLILLLSWVG HVKPQALDSG541QGAALTTATQ TTHLELQRGR QVTVPRAWLL FLRGSLPTFR SSLFLWVRPN GRLGPLVAGR601RPSALSQGRG QDLLSTVSIR YSGHSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
626 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.