Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GORASP2 / GRASP55 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101330-100
Catalog Number: LS-G101330
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GORASP2 (NM_015530), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Golgi phosphoprotein 6 | GRASP55 | GRS2 | GORASP2 | p59 | GOLPH6
- Origin Species
- Human
- NCBI GenBank
- NM_015530
- SwissProt
- Q9H8Y8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSINGSRLNK DNDTLKDLLK61ANVEKPVKML IYSSKTLELR ETSVTPSNLW GGQGLLGVSI RFCSFDGANE NVWHVLEVES121NSPAALAGLR PHSDYIIGAD TVMNESEDLF SLIETHEAKP LKLYVYNTDT DNCREVIITP181NSAWGGEGSL GCGIGYGYLH RIPTRPFEEG KKISLPGQMA GTPITPLKDG FTEVQLSSVN241PPSLSPPGTT GIEQSLTGLS ISSTPPAVSS VLSTGVPTVP LLPPQVNQSL TSVPPMNPAT301TLPGLMPLPA GLPNLPNLNL NLPAPHIMPG VGLPELVNPG LPPLPSMPPR NLPGIAPLPL361PSEFLPSFPL VPESSSAASS GELLSSLPPT SNAPSDPATT TAKADAASSL TVDVTPPTAK421APTTVEDRVG DSTPVSEKPV SAAVDANASE SPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
452 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.