Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GORASP1 / GRASP65 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102011-100
Catalog Number: LS-G102011
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRASP65(GORASP1) (NM_031899), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GORASP1 | Golgi phosphoprotein 5 | GRASP65 | GOLPH5 | p65
- Origin Species
- Human
- NCBI GenBank
- NM_031899
- SwissProt
- Q9BQQ3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLGVSAEQP AGGAEGFHLH GVQENSPAQQ AGLEPYFDFI ITIGHSRLNK ENDTLKALLK61ANVEKPVKLE VFNMKTMRVR EVEVVPSNMW GGQGLLGASV RFCSFRRASE QVWHVLDVEP121SSPAALAGLR PYTDYVVGSD QILQESEDFF TLIESHEGKP LKLMVYNSKS DSCREVTVTP181NAAWGGEGSL GCGIGYGYLH RIPTQPPSYH KKPPGTPPPS ALPLGAPPPD ALPPGPTPED241SPSLETGSRQ SDYMEALLQA PGSSMEDPLP GPGSPSHSAP DPDGLPHFME TPLQPPPPVQ301RVMDPGFLDV SGISLLDNSN ASVWPSLPSS TELTTTAVST SGPEDICSSS SSHERGGEAT361WSGSEFEVSF LDSPGAQAQA DHLPQLTLPD SLTSAASPED GLSAELLEAQ AEEEPASTEG421LDTGTEAEGL DSQAQISTTETRTRPLEQKLISEEDLAANDILDYKDDDDKV
440 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.