Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GORAB / SCYL1BP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106422-100
Catalog Number: LS-G106422
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCYL1BP1(GORAB) (NM_152281), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Golgin | RAB6-interacting | GO | NTKL-BP1 | RAB6-interacting golgin | NTKLBP1 | SCY1-like 1-binding protein 1 | SCYL1-BP1 | SCYL1BP1 | GORAB | HNTKL-BP1 | NTKL-binding protein 1 | SCY1-like 1 binding protein 1 | SCYL1-binding protein 1
- Origin Species
- Human
- NCBI GenBank
- AK021814
- SwissProt
- Q5T7V8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSWAAVLAVA AARFGHFWGC RWPGPMAQGW AGFSEEELRR LKQTKDPFEP QRRLPAKKSR61QQLQREKALV EQSQKLGLQD GSTSLLPEQL LSAPKQRVNV QKPPFSSPTL PSHFTLTSPV121GDGQPQGIES QPKELGLENS HDGHNNVEIL PPKPDCKLEK KKVELQEKSR WEVLQQEQRL181MEEKNKRKKA LLAKAIAERS KRTQAETMKL KRIQKELQAL DDMVSADIGI LRNRIDQASL241DYSYARKRFD RAEAEYIAAK LDIQRKTEIK EQLTEHLCTI IQQNELRKAK KLEELMQQLD301VEADEETLEL EVEVERLLHE QEVESRRPVV RLERPFQPAE ESVTLEFAKE NRKCQEQAVS361PKVDDQCGNS SSIPFLSPNC PNQEGNDISA ALATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
394 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.