Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GOLM1 / GP73 / GOLPH2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104835-100
Catalog Number: LS-G104835
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GOLPH2(GOLM1) (NM_177937), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA379P1.3 | Golgi membrane protein GP73 | Golgi phosphoprotein 2 | Golgi protein | 73-kD | gp73 | GOLPH2 | PSEC0257 | C9orf155 | Golgi membrane protein 1 | GOLM1
- Origin Species
- Human
- NCBI GenBank
- NM_016548
- SwissProt
- Q8NBJ4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLGNGRRSM KSPPLVLAAL VACIIVLGFN YWIASSRSVD LQTRIMELEG RVRRAAAERG61AVELKKNEFQ GELEKQREQL DKIQSSHNFQ LESVNKLYQD EKAVLVNNIT TGERLIRVLQ121DQLKTLQRNY GRLQQDVLQF QKNQTNLERK FSYDLSQCIN QMKEVKEQCE ERIEEVTKKG181NEAVASRDLS ENNDQRQQLQ ALSEPQPRLQ AAGLPHTEVP QGKGNVLGNS KSQTPAPSSE241VVLDSKRQVE KEETNEIQVV NEEPQRDRLP QEPGREQVVE DRPVGGRGFG GAGELGQTPQ301VQAALSVSQE NPEMEGPERD QLVIPDGQEE EQEAAGEGRN QQKLRGEDDY NMDENEAESE361TDKQAALAGN DRNIDVFNVE DQKRDTINLL DQREKRNHTL TRTRPLEQKL ISEEDLAAND421ILDYKDDDDK V
431 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.