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GOLGA5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GOLGA5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G116166-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GOLGA5 (NM_005113), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Golgin A5 | Golgin subfamily A member 5 | GOLIM5 | GOLGA5 | RETII | Protein Ret-II | PTC5 | Ret-II | RFG5 | Golgin-84 | RET-fused gene 5 protein
Origin Species
Human
NCBI GenBank
NM_005113
SwissProt
Q8TBA6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSWFVDLAGK AEDLLNRVDQ GAATALSRKD NASNIYSKNT DYTELHQQNT DLIYQTGPKS
61TYISSAADNI RNQKATILAG TANVKVGSRT PVEASHPVEN ASVPRPSSHF VRRKKSEPDD
121ELLFDFLNSS QKEPTGRVEI RKEKGKTPVF QSSQTSSVSS VNPSVTTIKT IEENSFGSQT
181HEAASNSDSS HEGQEESSKE NVSSNAACPD HTPTPNDDGK SHELSNLRLE NQLLRNEVQS
241LNQEMASLLQ RSKETQEELN KARARVEKWN ADHSKSDRMT RGLRAQVDDL TEAVAAKDSQ
301LAVLKVRLQE ADQLLSTRTE ALEALQSEKS RIMQDQSEGN SLQNQALQTF QERLHEADAT
361LKREQESYKQ MQSEFAARLN KVEMERQNLA EAITLAERKY SDEKKRVDEL QQQVKLYKLN
421LESSKQELID YKQKATRILQ SKEKLINSLK EGSGFEGLDS STASSMELEE LRHEKEMQRE
481EIQKLMGQIH QLRSELQDME AQQVNEAESA REQLQDLHDQ IAGQKASKQE LETELERLKQ
541EFHYIEEDLY RTKNTLQSRI KDRDEEIQKL RNQLTNKTLS NSSQSELENR LHQLTETLIQ
601KQTMLESLST EKNSLVFQLE RLEQQMNSAS GSSSNGSSIN MSGIDNGEGT RLRNVPVLFN
661DTETNLAGMY GKVRKAASSI DQFSIRLGIF LRRYPIARVF VIIYMALLHL WVMIVLLTYT
721PEMHHDQPYG K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

731 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.