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GOLGA3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GOLGA3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115643-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GOLGA3 (NM_005895), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Golgin subfamily A member 3 | Golgin-160 | GOLGA3 | Male enhanced antigen-2 | Golgin-165 | MEA-2 | GCP170 | Golgin A3 | SY2/SY10 protein
Origin Species
Human
NCBI GenBank
AB027133
SwissProt
Q08378

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
167.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDGASAEQDG LQEDRSHSGP SSLPEAPLKP PGPLVPPDQQ DKVQCAEVNR ASTEGESPDG
61PGQGGLCQNG PTPPFPDPPS SLDPTTSPVG PDASPGVAGF HDNLRKSQGT SAEGSVRKEA
121LQSLRLSLPM QETQLCSTDS PLPLEKEEQV RLQARKWLEE QLKQYRVKRQ QERSSQPATK
181TRLFSTLDPE LMLNPENLPR ASTLAMTKEY SFLRTSVPRG PKVGSLGLPA HPREKKTSKS
241SKIRSLADYR TEDSNAGNSG GNVPAPDSTK GSLKQNRSSA ASVVSEISLS PDTDDRLENT
301SLAGDSVSEV DGNDSDSSSY SSASTRGTYG ILSKTVGTQD TPYMVNGQEI PADTLGQFPS
361IKDVLQAAAA EHQDQGQEVN GEVRSRRDSI CSSVSLESSA AETQEEMLQV LKEKMRLEGQ
421LEALSLEASQ ALKEKAELQA QLAALSTKLQ AQVECSHSSQ QRQDSLSSEV DTLKQSCWDL
481ERAMTDLQNM LEAKNASLAS SNNDLQVAEE QYQRLMAKVE DMQRSMLSKD NTVHDLRQQM
541TALQSQLQQV QLERTTLTSK LKASQAEISS LQSVRQWYQQ QLALAQEARV RLQGEMAHIQ
601VGQMTQAGLL EHLKLENVSL SQQLTETQHR SMKEKGRIAA QLQGIEADML DQEAAFMQIQ
661EAKTMVEEDL QRRLEEFEGE RERLQRMADS AASLEQQLEQ VKLTLLQRDQ QLEALQQEHL
721DLMKQLTLTQ EALQSREQSL DALQTHYDEL QARLGELQGE AASREDTICL LQNEKIILEA
781ALQAAKSGKE ELDRGARRLE EGTEETSETL EKLREELAIK SGQVEHLQQE TAALKKQMQK
841IKEQFLQQKV MVEAYRRDAT SKDQLISELK ATRKRLDSEL KELRQELMQV HGEKRTAEAE
901LSRLHREVAQ VRQHMADLEG HLQSAQKERD EMETHLQSLQ FDKEQMVAVT EANEALKKQI
961EELQQEARKA ITEQKQKMRR LGSDLTSAQK EMKTKHKAYE NAVGILSRRL QEALAAKEAA
1021DAELGQLRAQ GGSSDSSLAL HERIQALEAE LQAVSHSKTL LEKELQEVIA LTSQELEESR
1081EKVLELEDEL QESRGFRKKI KRLEESNKKL ALELEHEKGK LTGLGQSNAA LREHNSILET
1141ALAKREADLV QLNLQVQAVL QRKEEEDRQM KHLVQALQAS LEKEKEKVNS LKEQVAAAKV
1201EAGHNRRHFK AASLELSEVK KELQAKEHLV QKLQAEADDL QIREGKHSQE IAQFQAELAE
1261ARAQLQLLQK QLDEQLSKQP VGNQEMENLK WEVDQKEREI QSLKQQLDLT EQQGRKELEG
1321LQQLLQNVKS ELEMAQEDLS MTQKDKFMLQ AKVSELKNNM KTLLQQNQQL KLDLRRGAAK
1381TRKEPKGEAS SSNPATPIKI PDCPVPASLL EELLRPPPAV SKEPLKNLNS CLQQLKQEMD
1441SLQRQMEEHA LTVHESLSSW TPLEPATASP VPPGGHAGPR GDPQRHSQSR ASKEGPGE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1498 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.