Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GOLGA1 / Golgin-97 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118209-100
Catalog Number: LS-G118209
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Golgin 97(GOLGA1) (NM_002077), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Golgin subfamily A member 1 | GOLGA1 | Golgin-97 | Golgin A1
- Origin Species
- Human
- NCBI GenBank
- NM_002077
- SwissProt
- Q92805
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFAKLKKKIA EETAVAQRPG GATRIPRSVS KESVASMGAD SGDDFASDGS SSREDLSSQL61LRRNEQIRKL EARLSDYAEQ VRNLQKIKEK LEIALEKHQD SSMRKFQEQN ETFQANRAKM121AEGLALALAR KDQEWSEKMD QLEKEKNILT AQLQEMKNQS MNLFQRRDEM DELEGFQQQE181LSKIKHMLLK KEESLGKMEQ ELEARTRELS RTQEELMNSN QMSSDLSQKL EELQRHYSTL241EEQRDHVIAS KTGAESKITA LEQKEQELQA LIQQLSIDLQ KVTAETQEKE DVITHLQEKV301ASLEKRLEQN LSGEEHVQEL LKEKTLAEQN LEDTRQQLLA ARSSQAKAIN TLETRVRELE361QTLQASEEQL QQSKGIVAAQ ETQIQELAAA NQESSHVQQQ ALALEQQFLE RTQALEAQIV421ALERMRAADQ TTAEQGMRQL EQENAALKEC RNEYERSLQN HQFELKKLKE EWSQREIVSV481AMAQALEEVR KQREEFQQQA ANLTAIIDEK EQNLREKTEV LLQKEQEILQ LERGHNSALL541QIHQLQAELE ALRTLKAEEA AVVAEQEDLL RLRGPLQAEA LSVNESHVTS RAMQDPVFQL601PTAGRTPNGE VGAMDLTQLQ KEKQDLEQQL LEKNKTIKQM QQRMLELRKT LQKELKIRPD661NELFEVREKP GPEMANMAPS VTNNTDLTDA REINFEYLKH VVLKFMSCRE SEAFHLIKAV721SVLLNFSQEE ENMLKETLEY KMSWFGSKPA PKGSIRPSIS NPRIPWSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
767 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.