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GNL3L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GNL3L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111462-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GNL3L (NM_019067), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GNL3L | Novel GTPase | RP11-353K22.1
Origin Species
Human
NCBI GenBank
NM_019067
SwissProt
Q9NVN8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMKLRHKNKK PGEGSKGHKK ISWPYPQPAK QNGKKATSKV PSAPHFVHPN DHANREAELK
61KKWVEEMREK QQAAREQERQ KRRTIESYCQ DVLRRQEEFE HKEEVLQELN MFPQLDDEAT
121RKAYYKEFRK VVEYSDVILE VLDARDPLGC RCFQMEEAVL RAQGNKKLVL VLNKIDLVPK
181EVVEKWLDYL RNELPTVAFK ASTQHQVKNL NRCSVPVDQA SESLLKSKAC FGAENLMRVL
241GNYCRLGEVR THIRVGVVGL PNVGKSSLIN SLKRSRACSV GAVPGITKFM QEVYLDKFIR
301LLDAPGIVPG PNSEVGTILR NCVHVQKLAD PVTPVETILQ RCNLEEISNY YGVSGFQTTE
361HFLTAVAHRL GKKKKGGLYS QEQAAKAVLA DWVSGKISFY IPPPATHTLP THLSAEIVKE
421MTEVFDIEDT EQANEDTMEC LATGESDELL GDTDPLEMEI KLLHSPMTKI ADAIENKTTV
481YKIGDLTGYC TNPNRHQMGW AKRNVDHRPK SNSMVDVCSV DRRSVLQRIM ETDPLQQGQA
541LASALKNKKK MQKRADKIAS KLSDSMMSAL DLSGNADDGV GD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

582 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.