LSBio
GMEB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GMEB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GMEB2 antibodies →
$504.00each
Catalog Number: LS-G114488-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GMEB2 (NM_012384), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GMEB-2 | GMEB2 | KIAA1269 | PIF p79 | PIF79 | DNA-binding protein p79PIF | p79PIF
Origin Species
Human
NCBI GenBank
NM_012384
SwissProt
Q9UKD1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATPDVSVHM EEVVVVTTPD TAVDGSGVEG VKTVLVTTNL APHGGDLTED NMETENAAAA
61AAAAFTASSQ LKEAVLVKMA EEGENLEAEI VYPITCGDSR ANLIWRKFVC PGINVKCVQY
121DEHVISPKEF VHLAGKSTLK DWKRAIRMNG IMLRKIMDSG ELDFYQHDKV CSNTCRSTKI
181DLSGARVSLS SPTSAEYIPL TPAAADVNGS PATITIETCE DPGDWTAAIG DDTFTFWRGL
241KDAGLLDEVI QEFHQELVET MRGLQQRVQD PPLQLRDAVL LNNIVQNFGM LDLVKKVLAS
301HKCQMDRSRE QYARDLAALE QQCDEHRRRA KELKHKSQHL SNVLMTLTPV SLPPPVKRPR
361LARATSGPAA MASQVLTQSA QLALGPGVPV PQLTSVPLGK VVSTLPSTVL GKGSLQAPPA
421SSPASPLLGG YTVLASSGST YPSTVEIHPD ASSLTVLSTA AVQDGSTVFK VVSPLQLLTL
481PGLGPTLQNV AQASPGSSTI VTVPAGAAPG PEEHTATIEV AAMAEDHERK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

530 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.