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GMCL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GMCL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104706-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GMCL1 (NM_178439), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTBD13 | GCL | GCL1 | Germ cell-less protein-like 1 | GMCL1 | Germ cell-less homolog 1 | SPATA29
Origin Species
Human
NCBI GenBank
N49477

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSLSSRVLR QPRPALAQQA QGARAGGSAR RPDTGDDAAG HGFCYCAGSH KRKRSSGSFC
61YCHPDSETDE DEEEGDEQQR LLNTPRRKKL KSTSKYIYQT LFLNGENSDI KICALGEEWS
121LHKIYLCQSG YFSSMFSGSW KESSMNIIEL EIPDQNIDVE ALQVAFGSLY RDDVLIKPSR
181VVAILAAACL LQLDGLIQQC GETMKETVNV KTVCGYYTSA GTYGLDSVKK KCLEWLLNNL
241MTHQNVELFK ELSINVMKQL IGSSNLFVMQ VEMDIYTALK KWMFLQLVPS WNGSLKQLLT
301ETDVWFSKQR KDFEGMAFLE TEQGKPFVSV FRHLRLQYII SDLASARIIE QDAVVPSEWL
361SSVYKQQWFA MLRAEQDSEV GPQEINKEEL EGNSMRCGRK LAKDGEYCWR WTGFNFGFDL
421LVTYTNRYII FKRNTLNQPC SGSVSLQPRR SIAFRLRLAS FDSSGKLICS RTTGYQILTL
481EKDQEQVVMN LDSRLLIFPL YICCNFLYIS PEKKN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

515 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.