Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GLYCTK / Glycerate Kinase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106704-100
Catalog Number: LS-G106704
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLYCTK (NM_145262), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HBeAg binding protein 4 | HBeAg-binding protein 4 | Glycerate kinase | GLYCTK | GLYCTK1 | HBeAg-binding protein 2 | HBeAgBP4A | HBEBP2 | HBEBP4
- Origin Species
- Human
- NCBI GenBank
- N92672
- SwissProt
- Q8IVS8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAALQVLPR LARAPLHPLL WRGSVARLAS SMALAEQARQ LFESAVGAVL PGPMLHRALS61LDPGGRQLKV RDRNFQLRQN LYLVGFGKAV LGMAAAAEEL LGQHLVQGVI SVPKGIRAAM121ERAGKQEMLL KPHSRVQVFE GAEDNLPDRD ALRAALAIQQ LAEGLTADDL LLVLISGGGS181ALLPAPIPPV TLEEKQTLTR LLAARGATIQ ELNTIRKALS QLKGGGLAQA AYPAQVVSLI241LSDVVGDPVE VIASGPTVAS SHNVQDCLHI LNRYGLRAAL PRSVKTVLSR ADSDPHGPHT301CGHVLNVIIG SNVLALAEAQ RQAEALGYQA VVLSAAMQGD VKSMAQFYGL LAHVARTRLT361PSMAGASVEE DAQLHELAAE LQIPDLQLEE ALETMAWGRG PVCLLAGGEP TVQLQGSGRG421GRNQELALRV GAELRRWPLG PIDVLFLSGG TDGQDGPTEA AGAWVTPELA SQAAAEGLDI481ATFLAHNDSH TFFCCLQGGA HLLHTGMTGT NVMDTHLLFL RPRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
523 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.