Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GLTSCR1L / KIAA0240 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113279-100
Catalog Number: LS-G113279
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0240(GLTSCR1L) (NM_015349), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0240 | GLTSCR1-like | GLTSCR1L
- Origin Species
- Human
- NCBI GenBank
- D87077
- SwissProt
- Q6AI39
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 114.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDDDDDSCLL DLIGDPQALN YFLHGPSNKS SNDDLTNAGY SAANSNSIFA NSSNADPKSS61LKGVSNQLGE GPSDGLPLSS SLQFLEDELE SSPLPDLTED QPFDILQKSL QEANITEQTL121AEEAYLDASI GSSQQFAQAQ LHPSSSASFT QASNVSNYSG QTLQPIGVTH VPVGASFASN181TVGVQHGFMQ HVGISVPSQH LSNSSQISGS GQIQLIGSFG NHPSMMTINN LDGSQIILKG241SGQQAPSNVS GGLLVHRQTP NGNSLFGNSS SSPVAQPVTV PFNSTNFQTS LPVHNIIIQR301GLAPNSNKVP INIQPKPIQM GQQNTYNVNN LGIQQHHVQQ GISFASASSP QGSVVGPHMS361VNIVNQQNTR KPVTSQAVSS TGGSIVIHSP MGQPHAPQSQ FLIPTSLSVS SNSVHHVQTI421NGQLLQTQPS QLISGQVASE HVMLNRNSSN MLRTNQPYTG PMLNNQNTAV HLVSGQTFAA481SGSPVIANHA SPQLVGGQMP LQQASPTVLH LSPGQSSVSQ GRPGFATMPS VTSMSGPSRF541PAVSSASTAH PSLGSAVQSG SSGSNFTGDQ LTQPNRTPVP VSVSHRLPVS SSKSTSTFSN601TPGTGTQQQF FCQAQKKCLN QTSPISAPKT TDGLRQAQIP GLLSTTLPGQ DSGSKVISAS661LGTAQPQQEK VVGSSPGHPA VQVESHSGGQ KRPAAKQLTK GAFILQQLQR DQAHTVTPDK721SHFRSLSDAV QRLLSYHVCQ GSMPTEEDLR KVDNEFETVA TQLLKRTQAM LNKYRCLLLE781DAMRINPSAE MVMIDRMFNQ EERASLSRDK RLALVDPEGF QADFCCSFKL DKAAHETQFG841RSDQHGSKAS SSLQPPAKAQ GRDRAKTGVT EPMNHDQFHL VPNHIVVSAE GNISKKTECL901GRALKFDKVG LVQYQSTSEE KASRREPLKA SQCSPGPEGH RKTSSRSDHG TESKLSSILA961DSHLEMTCNN SFQDKSLRNS PKNEVLHTDI MKGSGEPQPD LQLTKSLETT FKNILELKKA1021GRQPQSDPTV SGSVELDFPN FSPMASQENC LEKFIPDHSE GVVETDSILE AAVNSILECTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1079 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.