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GLI4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GLI4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107301-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLI4 (NM_138465), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLI family zinc finger 4 | GLI-Kruppel family member GLI4 | HKR4 | ZNF928 | Protein HKR4 | Zinc finger protein GLI4 | GLI4
Origin Species
Human
NCBI GenBank
M20678
SwissProt
P10075

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
41 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAALGDIQES PSVPSPVSLS SPGTPGTQHH EPQLHLHGHQ HGSPGSSPKV LSQPSDLDLQ
61DVEEVEIGRD TFWPDSEPKP EQAPRSPGSQ APDEGAGGAL RSLLRSLPRR ARCSAGFGPE
121SSAERPAGQP PGAVPCAQPR GAWRVTLVQQ AAAGPEGAPE RAAELGVNFG RSRQGSARGA
181KPHRCEACGK SFKYNSLLLK HQRIHTGEKP YACHECGKRF RGWSGFIQHH RIHTGEKPYE
241CGQCGRAFSH SSHFTQHLRI HNGEKPYKCG ECGQAFSQSS NLVRHQRLHT GEKPYACSQC
301GKAFIWSSVL IEHQRIHTGE KPYECSDCGK AFRGRSHFFR HLRTHTGEKP FACGACGKAF
361GQSSQLIQHQ RVHYRE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

376 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.