Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GLDN / Gliomedin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104380-100
Catalog Number: LS-G104380
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Gliomedin(GLDN) (NM_181789), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Colmedin | CRG-L2 | CLOM | COLM | CRGL2 | Gliomedin | GLDN | UNC-112 | Collomin
- Origin Species
- Human
- NCBI GenBank
- NM_181789
- SwissProt
- Q6ZMI3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARGAEGGRG DAGWGLRGAL AAVALLSALN AAGTVFALCQ WRGLSSALRA LEAQRGREQR61EDSALRSFLA ELSRAPRGAS APPQDPASSA RNKRSHSGEP APHIRAESHD MLMMMTYSMV121PIRVMVDLCN STKGICLTGP SGPPGPPGAG GLPGHNGLDG QPGPQGPKGE KGANGKRGKM181GIPGAAGNPG ERGEKGDHGE LGLQGNEGPP GQKGEKGDKG DVSNDVLLAG AKGDQGPPGP241PGPPGPPGPP GPPGSRRAKG PRQPSMFNGQ CPGETCAIPN DDTLVGKADE KASEHHSPQA301ESMITSIGNP VQVLKVTETF GTWIRESANK SDDRIWVTEH FSGIMVKEFK DQPSLLNGSY361TFIHLPYYFH GCGHVVYNNS LYYHKGGSNT LVRFEFGQET SQTLKLENAL YFDRKYLFAN421SKTYFNLAVD EKGLWIIYAS SVDGSSILVA QLDERTFSVV QHVNTTYPKS KAGNAFIARG481ILYVTDTKDM RVTFAFDLLG GKQINANFDL RTSQSVLAML AYNMRDQHLY SWEDGHLMLY541PVQFLSTTLN QTRTRPLEQKLISEEDLAANDILDYKDDDDKV
551 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.