Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GLCNE / GNE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G131054-100
Catalog Number: LS-G131054
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLCNE(GNE) (NM_001190384), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DMRV | GNE | IBM2 | GLCNE | N-acylmannosamine kinase | NM | Uae1
- Origin Species
- Human
- NCBI GenBank
- NM_005476
- SwissProt
- Q9Y223
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIEQDDFDIN TRLHTIVRGE DEAAMVESVG LALVKLPDVL NRLKPDIMIV HGDRFDALAL61ATSAALMNIR ILHIEGGEVS GTIDDSIRHA ITKLAHYHVC CTRSAEQHLI SMCEDHDRIL121LAGCPSYDKL LSAKNKDYMS IIRMWLGSKE MVRVMRKKGI EHHPNFRAVK HVPFDQFIQL181VAHAGCMIGN SSCGVREVGA FGTPVINLGT RQIGRETGEN VLHVRDADTQ DKILQALHLQ241FGKQYPCSKI YGDGNAVPRI LKFLKSIDLQ EPLQKKFCFP PVKENISQDI DHILETLSAL301AVDLGGTNLR VAIVSMKGEI VKKYTQFNPK TYEERINLIL QMCVEAAAEA VKLNCRILGV361GISTGGRVNP REGIVLHSTK LIQEWNSVDL RTPLSDTLHL PVWVDNDGNC AALAERKFGQ421GKGLENFVTL ITGTGIGGGI IHQHELIHGS SFCAAELGHL VVSLDGPDCS CGSHGCIEAY481ASGMALQREA KKLHDEDLLL VEGMSVPKDE AVGALHLIQA AKLGNAKAQS ILRTAGTALG541LGVVNILHTM NPSLVILSGV LASHYIHIVK DVIRQQALSS VQDVDVVVSD LVDPALLGAA601SMVLDYTTRR IYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
612 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.