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GLB1L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GLB1L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GLB1L3 antibodies →
$725.00each
Catalog Number: LS-G120194-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLB1L3 (NM_001080407), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Galactosidase | beta 1 like 3 | beta 1-like 3 | GLB1L3
Origin Species
Human
SwissProt
Q8NCI6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKSPPLLSPC LSWKRMAGIF FLPFISSGFA PRFKQEENFM LGRAHPSQPR FNWSHLTPLE
61LKNRSVGLGT ESTGRGKPHF TLEGHKFLIF GGSIHYFRVP REYWRDRLLK LKACGFNTVT
121TYVPWNLHEP ERGKFDFSGN LDLEAFVLMA AEIGLWVILR PGRYICSEMD LGGLPSWLLQ
181DPRLLLRTTN KSFIEAVEKY FDHLIPRVIP LQYRQAGPVI AVQVENEYGS FNKDKTYMPY
241LHKALLRRGI VELLLTSDGE KHVLSGHTKG VLAAINLQKL HQDTFNQLHK VQRDKPLLIM
301EYWVGWFDRW GDKHHVKDAK EVEHAVSEFI KYEISFNVYM FHGGTNFGFM NGATYFGKHS
361GIVTSYDYDA VLTEAGDYTE KYLKLQKLFQ SVSATPLPRV PKLPPKAVYP PVRPSLYLPL
421WDALSYLNEP VRSRQPVNME NLPINNGSGQ SYGLVLYEKS ICSGGRLRAH AHDVAQVFLD
481ETMIGILNEN NKDLHIPELR DCRYLRILVE NQGRVNFSWQ IQNEQKGITG SVSINNSSLE
541GFTIYSLEMK MSFFERLRSA TWKPVPDSHQ GPAFYCGTLK AGPSPKDTFL SLLNWNYGFV
601FINGRNLGRY WNIGPQKTLY LPGVWLHPED NEVILFEKMM SGSDIKSTDK PTL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

653 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.