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GLB1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GLB1L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GLB1L antibodies →
$725.00each
Catalog Number: LS-G109942-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLB1L (NM_024506), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLB1L | Galactosidase | beta 1-like
Origin Species
Human
NCBI GenBank
NM_024506
SwissProt
Q6UWU2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPKKLSCLR SLLLPLSLTL LLPQADTRSF VVDRGHDRFL LDGAPFRYVS GSLHYFRVPR
61VLWADRLLKM RWSGLNAIQF YVPWNYHEPQ PGVYNFNGSR DLIAFLNEAA LANLLVILRP
121GPYICAEWEM GGLPSWLLRK PEIHLRTSDP DFLAAVDSWF KVLLPKIYPW LYHNGGNIIS
181IQVENEYGSY RACDFSYMRH LAGLFRALLG EKILLFTTDG PEGLKCGSLR GLYTTVDFGP
241ADNMTKIFTL LRKYEPHGPL VNSEYYTGWL DYWGQNHSTR SVSAVTKGLE NMLKLGASVN
301MYMFHGGTNF GYWNGADKKG RFLPITTSYD YDAPISEAGD PTPKLFALRD VISKFQEVPL
361GPLPPPSPKM MLGPVTLHLV GHLLAFLDLL CPRGPIHSIL PMTFEAVKQD HGFMLYRTYM
421THTIFEPTPF WVPNNGVHDR AYVMVDGVFQ GVVERNMRDK LFLTGKLGSK LDILVENMGR
481LSFGSNSSDF KGLLKPPILG QTILTQWMMF PLKIDNLVKW WFPLQLPKWP YPQAPSGPTF
541YSKTFPILGS VGDTFLYLPG WTKGQVWING FNLGRYWTKQ GPQQTLYVPR FLLFPRGALN
601KITLLELEDV PLQPQVQFLD KPILNSTSTL HRTHINSLSA DTLSASEPME LSGH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

654 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.