Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GJA9 / CX59 / Connexin 59 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109414-100
Catalog Number: LS-G109414
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GJA9(GJA9) (NM_030772), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Connexin 59 | Connexin-58 | Connexin-59 | CX58 | CX59 | Gap junction alpha 10 | Gap junction alpha-9 protein | Gap junction alpha-10 protein | Gap junction protein alpha-10 | GJA9
- Origin Species
- Human
- NCBI GenBank
- AF179597
- SwissProt
- P57773
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDWNLLGDT LEEVHIHSTM IGKIWLTILF IFRMLVLGVA AEDVWNDEQS GFICNTEQPG61CRNVCYDQAF PISLIRYWVL QVIFVSSPSL VYMGHALYRL RVLEEERQRM KAQLRVELEE121VEFEMPRDRR RLEQELCQLE KRKLNKAPLR GTLLCTYVIH IFTRSVVEVG FMIGQYLLYG181FHLEPLFKCH GHPCPNIIDC FVSRPTEKTI FLLFMQSIAT ISLFLNILEI FHLGFKKIKR241GLWGKYKLKK EHNEFHANKA KQNVAKYQST SANSLKRLPS APDYNLLVEK QTHTAVYPSL301NSSSVFQPNP DNHSVNDEKC ILDEQETVLS NEISTLSTSC SHFQHISSNN NKDTHKIFGK361ELNGNQLMEK RETEGKDSKR NYYSRGHRSI PGVAIDGENN MRQSPQTVFS LPANCDWKPR421WLRATWGSST EHENRGSPPK GNLKGQFRKG TVRTLPPSQG DSQSLDIPNT ADSLGGLSFE481PGLVRTCNNP VCPPNHVVSL TNNLIGRRVP TDLQITRTRPLEQKLISEEDLAANDILDYKDDDDKV
515 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.