Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GJA10 / CX62 / Connexin 62 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108694-100
Catalog Number: LS-G108694
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GJA10 (NM_032602), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Connexin-62 | Connexin 62 | CX62 | Gap junction alpha-10 protein | RP11-63K6.6 | GJA10
- Origin Species
- Human
- NCBI GenBank
- NM_032602
- SwissProt
- Q969M2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDWNLLGGI LEEVHSHSTI VGKIWLTILF IFRMLVLRVA AEDVWDDEQS AFACNTRQPG61CNNICYDDAF PISLIRFWVL QIIFVSSPSL VYMGHALYRL RAFEKDRQRK KSHLRAQMEN121PDLDLEEQQR IDRELRRLEE QKRIHKVPLK GCLLRTYVLH ILTRSVLEVG FMIGQYILYG181FQMHPLYKCT QPPCPNAVDC FVSRPTEKTI FMLFMHSIAA ISLLLNILEI FHLGIRKIMR241TLYKKSSSEG IEDETGPPFH LKKYSVAQQC MICSSLPERI SPLQANNQQQ VIRVNVPKSK301TMWQIPQPRQ LEVDPSNGKK DWSEKDQHSG QLHVHSPCPW AGSAGNQHLG QQSDHSSFGL361QNTMSQSWLG TTTAPRNCPS FAVGTWEQSQ DPEPSGEPLT DLHSHCRDSE GSMRESGVWI421DRSRPGSRKA SFLSRLLSEK RHLHSDSGSS GSRNSSCLDF PHWENSPSPL PSVTGHRTSM481VRQAALPIME LSQELFHSGC FLFPFFLPGV CMYVCVDREA DGGGDYLWRD KIIHSIHSVK541FNSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
543 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.