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GIT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GIT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G125437-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GIT2 (NM_001135213), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CAT2 | KIAA0148 | GIT2 | GRK-interacting protein 2 | ARF GAP GIT2 | CAT-2
Origin Species
Human
NCBI GenBank
NM_014776
SwissProt
Q14161

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL
61LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL
121PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI
181LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD
241HKNGQHFIIP QMADRRLSLD LSELAKAAKK KLQSLSNHLF EELAMDVYDE VDRRETDAVW
301LATQNHSALV TETTVVPFLP VNPEYSSTRN QGRQKLARFN AHEFATLVID ILSDAKRRQQ
361GSSLSGSKDN VELILKTINN QHSVESQDND QPDYDSVASD EDTDLETTAS KTNRQKLQTL
421QSENSNLRKQ ATTNVYQVQT GSEYTDTSNH SSLKRRPSAR GSRPMSMYET GSGQKPYLPM
481GEASRPEESR MRLQPFPAHA SRLEKQNSTP ESDYDNTPND MEPDGMGSSR KGRQRSMVWP
541GDGLVPDTAE PHVAPSPTLP STEDVIRKTE QITKNIQELL RAAQENKHDS YIPCSERIHV
601AVTEMAALFP KKPKSDMVRT SLRLLTSSAY RLQSECKKTL PGDPGSPTDV QLVTQQVIQC
661AYDIAKAAKQ LVTITTKENN N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

681 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.