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GIMAP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GIMAP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105021-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GIMAP8 (NM_175571), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GIMAP8 | GTPase | IMAP family member 8 | HIAN6 | IAN-9 | IANT | Immune associated nucleotide | IAN6 | Protein IanT | GTPase IMAP family member 8 | IAN9
Origin Species
Human
NCBI GenBank
NM_175571
SwissProt
Q8ND71

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEQSCQMSE LRLLLLGKCR SGKSATGNAI LGKHVFKSKF SDQTVIKMCQ RESWVLRERK
61VVVIDTPDLF SSIACAEDKQ RNIQHCLELS APSLHALLLV IAIGHFTRED EETAKGIQQV
121FGAEARRHII IVFTRKDDLG DDLLQDFIEK NKPLKQLVQD YEGRYCIFNN KTNSKDEQIT
181QVLELLRKVE SLVNTNGGPY HVNFKTEGSR FQDCVNEAAS QEGDKPQGPR ERQLQSTGPE
241QNPGTSELTV LLVGKRGAGK SAAGNSILGR QAFQTGFSEQ SVTQSFLSES RSWRKKKVSI
301IDAPDISSLK NIDSEVRKHI CTGPHAFLLV TPLGFYTKND EAVLSTIQNN FGEKFFEYMI
361ILLTRKEDLG DQDLDTFLRN SNKALYGLIQ KCKNRYSAFN YRATGEEEQR QADELLEKIE
421SMVHQNGNKH CVFREKETLN IVLVGRSGTG KSATGNSILG SLVFTSRLRA QPVTKTSQSG
481RRTWDGQEVV VVDTPSFNQM LDVEKDPSRL EEEVKRCLSC CEKGDTFFVL VFQLGRFTEE
541DKTAVAKLEA IFGADFTKYA IMLFTRKEDL GAGNLEDFMK NSDNKALRRI FKKCGRRVCA
601FNNKETGQAQ ETQVKALLTK VNDLRKESGW SGYPHTQENV SKLIKNVQEM SQAEKLLKNL
661IGILQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

665 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.