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GIGYF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GIGYF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101815-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GIGYF1 (NM_022574), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GYF1 | PERQ1 | GIGYF1 | GYF domain containing 1
Origin Species
Human
NCBI GenBank
NM_022574
SwissProt
O75420

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
114.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAETLNFGP EWLRALSGGG SVASPPPSPA MPKYKLADYR YGREEMLALY VKENKVPEEL
61QDKEFAAVLQ DEPLQPLALE PLTEEEQRNF SLSVNSVAVL RLMGKGAGPP LAGTSRGRGS
121TRSRGRGRGD SCFYQRSIEE GDGAFGRSPR EIQRSQSWDD RGERRFEKSA RRDGARCGFE
181EGGAGPRKEH ARSDSENWRS LREEQEEEEE GSWRLGAGPR RDGDRWRSAS PDGGPRSAGW
241REHGERRRKF EFDLRGDRGG CGEEEGRGGG GSSHLRRCRA PEGFEEDKDG LPEWCLDDED
301EEMGTFDASG AFLPLKKGPK EPIPEEQELD FQGLEEEEEP SEGLEEEGPE AGGKELTPLP
361PQEEKSSSPS PLPTLGPLWG TNGDGDETAE KEPPAAEDDI RGIQLSPGVG SSAGPPGDLE
421DDEGLKHLQQ EAEKLVASLQ DSSLEEEQFT AAMQTQGLRH SAAATALPLS HGAARKWFYK
481DPQGEIQGPF TTQEMAEWFQ AGYFSMSLLV KRGCDEGFQP LGEVIKMWGR VPFAPGPSPP
541PLLGNMDQER LKKQQELAAA ALYQQLQHQQ FLQLVSSRQL PQCALREKAA LGDLTPPPPP
601PPQQQQQQLT AFLQQLQALK PPRGGDQNLL PTMSRSLSVP DSGRLWDVHT SASSQSGGEA
661SLWDIPINSS TQGPILEQLQ LQHKFQERRE VELRAKREEE ERKRREEKRR QQQQEEQKRR
721QEEEELFRRK HVRQQELLLK LLQQQQAVPV PPAPSSPPPL WAGLAKQGLS MKTLLELQLE
781GERQLHKQPP PREPARAQAP NHRVQLGGLG TAPLNQWVSE AGPLWGGPDK SGGGSSGLGL
841WEDTPKSGGS LVRGLGLKNS RSSPSLSDSY SHLSGRPIRK KTEEEEKLLK LLQGIPRPQD
901GFTQWCEQML HTLSATGSLD VPMAVAILKE VESPYDVHDY IRSCLGDTLE AKEFAKQFLE
961RRAKQKASQQ RQQQQEAWLS SASLQTAFQA NHSTKLGPGE GSKAKRRALM LHSDPSILGY
1021SLHGSSGEIE SVDDY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1035 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.