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GHDC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GHDC Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126053-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GHDC (NM_001142623), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D11LGP1E | D11LGP1 | GHDC | Homolog of mouse LGP1 | LGP1 | GH3 domain containing | GH3 domain-containing protein
Origin Species
Human
NCBI GenBank
NM_032484
SwissProt
Q8N2G8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MLLWPLLLLL LLLPTLALLR QQRSQDARLS WLAGLQHRVA WGALVWAATW QRRRLEQSTL
61HVHQSQQQAL RWCLQGAQRP HCSLRRSTDI STFRNHLPLT KASQTQQEDS GEQPLPPTSN
121QDLGEASLQA TLLGLAALNK AYPEVLAQGR TARVTLTSPW PRPLPWPGNT LGQVGTPGTK
181DPRALLLDAL RSPGLRALEA GTAVELLDVF LGLETDGEEL AGAIAAGNPG APLRERAAEL
241REALEQGPRG LALRLWPKLQ VVVTLDAGGQ AEAVAALGAL WCQGLAFFSP AYAASGGVLG
301LNLQPEQPHG LYLLPPGAPF IELLPVKEGT QEEAASTLLL AEAQQGKEYE LVLTDRASLT
361RCRLGDVVRV VGAYNQCPVV RFICRLDQTL SVRGEDIGED LFSEALGRAV GQWAGAKLLD
421HGCVESSILE WGRTDRAEDT RLGSCPFTGS MCAHGVYSQI PLRALLPTTR CLWR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

474 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.