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GGA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GGA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113514-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GGA2 (NM_015044), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1080 | GGA2 | VEAR
Origin Species
Human
NCBI GenBank
NM_015044
SwissProt
Q9UJY4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67 kda
Protein Type
Lysate
Amino Acid Sequence
1MAATAVAAAV AGTESAQGPP GPAASLELWL NKATDPSMSE QDWSAIQNFC EQVNTDPNGP
61THAPWLLAHK IQSPQEKEAL YALTVLEMCM NHCGEKFHSE VAKFRFLNEL IKVLSPKYLG
121SWATGKVKGR VIEILFSWTV WFPEDIKIRD AYQMLKKQGI IKQDPKLPVD KILPPPSPWP
181KSSIFDADEE KSKLLTRLLK SNHPEDLQAA NRLIKNLVKE EQEKSEKVSK RVSAVEEVRS
241HVKVLQEMLS MYRRPGQAPP DQEALQVVYE RCEKLRPTLF RLASDTTDDD DALAEILQAN
301DLLTQGVLLY KQVMEGRVTF GNRVTSSLGD IPVSRVFQNP AGCMKTCPLI DLEVDNGPAQ
361MGTVVPSLLH QDLAALGISD APVTGMVSGQ NCCEEKRNPS SSTLPGGGVQ NPSADRNLLD
421LLSPQPAPCP LNYVSQKSVP KEVPPGTKSS PGWSWEAGPL APSPSSQNTP LAQVFVPLES
481VKPSSLPPLI VYDRNGFRIL LHFSQTGAPG HPEVQVLLLT MMSTAPQPVW DIMFQVAVPK
541SMRVKLQPAS SSKLPAFSPL MPPAVISQML LLDNPHKEPI RLRYKLTFNQ GGQPFSEVGE
601VKDFPDLAVL GAATRTRPLE QKLISEEDLA ANDILDYKDD DDKV

644 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.