Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GFRA1 / GFR Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126725-100
Catalog Number: LS-G126725
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GDNF Receptor alpha 1(GFRA1) (NM_001145453), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GDNF receptor alpha-1 | GDNFRA | GFR-ALPHA-1 | GFRA1 | GDNF family receptor alpha-1 | GDNFR | GDNFR-alpha-1 | RET ligand 1 | RETL1 | TRNR1 | RET1L | GDNF family receptor alpha 1 | GPI-linked anchor protein
- Origin Species
- Human
- NCBI GenBank
- NM_005264
- SwissProt
- P56159
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.84 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFLATLYFAL PLLDLLLSAE VSGGDRLDCV KASDQCLKEQ SCSTKYRTLR QCVAGKETNF61SLASGLEAKD ECRSAMEALK QKSLYNCRCK RGMKKEKNCL RIYWSMYQSL QGNDLLEDSP121YEPVNSRLSD IFRVVPFISV EHIPKGNNCL DAAKACNLDD ICKKYRSAYI TPCTTSVSND181VCNRRKCHKA LRQFFDKVPA KHSYGMLFCS CRDIACTERR RQTIVPVCSY EEREKPNCLN241LQDSCKTNYI CRSRLADFFT NCQPESRSVS SCLKENYADC LLAYSGLIGT VMTPNYIDSS301SLSVAPWCDC SNSGNDLEEC LKFLNFFKDN TCLKNAIQAF GNGSDVTVWQ PAFPVQTTTA361TTTTALRVKN KPLGPAGSEN EIPTHVLPPC ANLQAQKLKS NVSGNTHLCI SNGNYEKEGL421GASSHITTKS MAAPPSCGLS PLLVLVVTAL STLLSLTETSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
460 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.