Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GDPGP1 / C15orf58 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121526-100
Catalog Number: LS-G121526
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GDPGP1 (NM_001013657), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001013657
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MALPHDSNET SYLLPPNNED WGRQTIPDFV YGQKDLMAEG IQWPRNAPGI PDALPQSPFD61AALCSAWKQR VELGLFRYRL RELQTQILPG AVGFVAQLNV ERGVQRRPPQ TIKSVRQAFD121PVQFNFNKIR PGEVLFRLHR EPDLPGTLLQ EDILVVINVS PLEWGHVLLV PEPARQLPQR181LLPGALRAGI EAVLLSLHPG FRVGFNSLGG LASVNHLHLH GYYLAHRLPV EQAPSEPLDP241GGHLHLLQDL PAPGFLFYTR GPGPDLESLI SRVCRATDYL TDHEIAHNLF VTRGAPPGKT301SPSSALTGVR VILWARKSSF GIKDGEAFNV ALCELAGHLP VKTSQDFSSL TEAAAVALIQ361DCRLPPSQAE DVQAALVALM SQEEQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
385 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.