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GDPD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GDPD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109406-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GDPD5 (NM_030792), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GDE2 | GDPD5 | PP1665
Origin Species
Human
NCBI GenBank
NM_030792
SwissProt
Q8WTR4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVRHQPLQYY EPQLCLSCLT GIYGCRWKRY QRSHDDTTPW ERLWFLLLTF TFGLTLTWLY
61FWWEVHNDYD EFNWYLYNRM GYWSDWPVPI LVTTAAAFAY IAGLLVLALC HIAVGQQMNL
121HWLHKIGLVV ILASTVVAMS AVAQLWEDEW EVLLISLQGT APFLHVGAVA AVTMLSWIVA
181GQFARAERTS SQVTILCTFF TVVFALYLAP LTISSPCIME KKDLGPKPAL IGHRGAPMLA
241PEHTLMSFRK ALEQKLYGLQ ADITISLDGV PFLMHDTTLR RTTNVEEEFP ELARRPASML
301NWTTLQRLNA GQWFLKTDPF WTASSLSPSD HREAQNQSIC SLAELLELAK GNATLLLNLR
361DPPREHPYRS SFINVTLEAV LHSGFPQHQV MWLPSRQRPL VRKVAPGFQQ TSGSKEAVAS
421LRRGHIQRLN LRYTQVSRQE LRDYASWNLS VNLYTVNAPW LFSLLWCAGV PSVTSDNSHA
481LSQVPSPLWI MPPDEYCLMW VTADLVSFTL IVGIFVLQKW RLGGIRSYNP EQIMLSAAVR
541RTSRDVSIMK EKLIFSEISD GVEVSDVLSV CSDNSYDTYA NSTATPVGPR GGGSHTKTLI
601ERSGRSGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

608 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.