Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GDPD2 / GDE3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112292-100
Catalog Number: LS-G112292
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GDPD2 (NM_017711), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GDPD2 | OBDPF | GDE3
- Origin Species
- Human
- NCBI GenBank
- NM_017711
- SwissProt
- Q9HCC8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAESPGCCSV WARCLHCLYS CHWRKCPRER MQTSKCDCIW FGLLFLTFLL SLSWLYIGLV61LLNDLHNFNE FLFRRWGHWM DWSLAFLLVI SLLVTYASLL LVLALLLRLC RQPLHLHSLH121KVLLLLIMLL VAAGLVGLDI QWQQEWHSLR VSLQATAPFL HIGAAAGIAL LAWPVADTFY181RIHRRGPKIL LLLLFFGVVL VIYLAPLCIS SPCIMEPRDL PPKPGLVGHR GAPMLAPENT241LMSLRKTAEC GATVFETDVM VSSDGVPFLM HDEHLSRTTN VASVFPTRIT AHSSDFSWTE301LKRLNAGSWF LERRPFWGAK PLAGPDQKEA ESQTVPALEE LLEEAAALNL SIMFDLRRPP361QNHTYYDTFV IQTLETVLNA RVPQAMVFWL PDEDRANVQR RAPGMRQIYG RQGGNRTERP421QFLNLPYQDL PLLDIKALHK DNVSVNLFVV NKPWLFSLLW CAGVDSVTTN DCQLLQQMRY481PIWLITPQTY LIIWVITNCV STMLLLWTFL LQRRFVKKRG KTGLETAVLL TRINNFMMETRTRPLEQKLISEEDLAANDILDYKDDDDKV
539 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.