Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GDF10 / BMP3B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116284-100
Catalog Number: LS-G116284
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GDF10 (NM_004962), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BMP-3b | Bone-inducing protein | BIP | BMP3B | Bone morphogenetic protein 3B | GDF-10 | GDF10
- Origin Species
- Human
- NCBI GenBank
- NM_004962
- SwissProt
- P55107
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAHVPARTSP GPGPQLLLLL LPLFLLLLRD VAGSHRAPAW SALPAAADGL QGDRDLQRHP61GDAAATLGPS AQDMVAVHMH RLYEKYSRQG ARPGGGNTVR SFRARLEVVD QKAVYFFNLT121SMQDSEMILT ATFHFYSEPP RWPRALEVLC KPRAKNASGR PLPLGPPTRQ HLLFRSLSQN181TATQGLLRGA MALAPPPRGL WQAKDISPIV KAARRDGELL LSAQLDSEER DPGVPRPSPY241APYILVYAND LAISEPNSVA VTLQRYDPFP AGDPEPRAAP NNSADPRVRR AAQATGPLQD301NELPGLDERP PRAHAQHFHK HQLWPSPFRA LKPRPGRKDR RKKGQEVFMA ASQVLDFDEK361TMQKARRKQW DEPRVCSRRY LKVDFADIGW NEWIISPKSF DAYYCAGACE FPMPKIVRPS421NHATIQSIVR AVGIIPGIPE PCCVPDKMNS LGVLFLDENR NVVLKVYPNM SVDTCACRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
478 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.