Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GCP3 / TUBGCP3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115378-100
Catalog Number: LS-G115378
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Gamma tubulin complex component 3(TUBGCP3) (NM_006322), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GCP3 | H104p | GCP-3 | HGrip104 | SPBC98 | Spc98p | TUBGCP3 | HGCP3 | HSpc98
- Origin Species
- Human
- NCBI GenBank
- NM_006322
- SwissProt
- Q96CW5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATPDQKSPN VLLQNLCCRI LGRSEADVAQ QFQYAVRVIG SNFAPTVERD EFLVAEKIKK61ELIRQRREAD AALFSELHRK LHSQGVLKNK WSILYLLLSL SEDPRRQPSK VSSYATLFAQ121ALPRDAHSTP YYYARPQTLP LSYQDRSAQS AQSSGSVGSS GISSIGLCAL SGPAPAPQSL181LPGQSNQAPG VGDCLRQQLG SRLAWTLTAN QPSSQATTSK GVPSAVSRNM TRSRREGDTG241GTMEITEAAL VRDILYVFQG IDGKNIKMNN TENCYKVEGK ANLSRSLRDT AVRLSELGWL301HNKIRRYTDQ RSLDRSFGLV GQSFCAALHQ ELREYYRLLS VLHSQLQLED DQGVNLGLES361SLTLRRLLVW TYDPKIRLKT LAALVDHCQG RKGGELASAV HAYTKTGDPY MRSLVQHILS421LVSHPVLSFL YRWIYDGELE DTYHEFFVAS DPTVKTDRLW HDKYTLRKSM IPSFMTMDQS481RKVLLIGKSI NFLHQVCHDQ TPTTKMIAVT KSAESPQDAA DLFTDLENAF QGKIDAAYFE541TSKYLLDVLN KKYSLLDHMQ AMRRYLLLGQ GDFIRHLMDL LKPELVRPAT TLYQHNLTGI601LETAVRATNA QFDSPEILRR LDVRLLEVSP GDTGWDVFSL DYHVDGPIAT VFTRECMSHY661LRVFNFLWRA KRMEYILTDI RKGHMCNAKL LRNMPEFSGV LHQCHILASE MVHFIHQMQY721YITFEVLECS WDELWNKVQQ AQDLDHIIAA HEVFLDTIIS RCLLDSDSRA LLNQLRAVFD781QIIELQNAQD AIYRAALEEL QRRLQFEEKK KQREIEGQWG VTAAEEEEEN KRIGEFKESI841PKMCSQLRIL THFYQGIVQQ FLVLLTTSSD ESLRFLSFRL DFNEHYKARE PRLRVSLGTR901GRRSSHTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
907 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.