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GCFC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GCFC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GCFC2 antibodies →
$504.00each
Catalog Number: LS-G117491-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C2orf3(GCFC2) (NM_003203), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAHRPKRTFR QRAADSSDSD GAEESPAEPG AARELPVPGS AEEEPPSGGG RAQVAGLPHR
61VRGPRGRGRV WASSRRATKA APRADEGSES RTLDVSTDEE DKIHHSSESK DDQGLSSDSS
121SSLGEKELSS TVKIPDAAFI QAARRKRELA RAQDDYISLD VQHTSSISGM KRESEDDPES
181EPDDHEKRIP FTLRPQTLRQ RMAEESISRN EETSEESQED EKQDTWEQQQ MRKAVKIIEE
241RDIDLSCGNG SSKVKKFDTS ISFPPVNLEI IKKQLNTRLT LLQETHRSHL REYEKYVQDV
301KSSKSTIQNL ESSSNQALNC KFYKSMKIYV ENLIDCLNEK IINIQEIESS MHALLLKQAM
361TFMKRRQDEL KHESTYLQQL SRKDETSTSG NFSVDEKTQW ILEEIESRRT KRRQARVLSG
421NCNHQEGTSS DDELPSAEMI DFQKSQGDIL QKQKKVFEEV QDDFCNIQNI LLKFQQWREK
481FPDSYYEAFI SLCIPKLLNP LIRVQLIDWN PLKLESTGLK EMPWFKSVEE FMDSSVEDSK
541KESSSDKKVL SAIINKTIIP RLTDFVEFLW DPLSTSQTTS LITHCRVILE EHSTCENEVS
601KSRQDLLKSI VSRMKKAVED DVFIPLYPKS AVENKTSPHS KFQERQFWSG LKLFRNILLW
661NGLLTDDTLQ ELGLGKLLNR YLIIALLNAT PGPDVVKKCN QVAACLPEKW FENSAMRTSI
721PQLENFIQFL LQSAHKLSRS EFRDEVEEII LILVKIKALN QAESFIGEHH LDHLKSLIKE
781D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

781 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.