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GBP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GBP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108079-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GBP5 (NM_052942), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GBP-TA antigen | GTP-binding protein 5 | Guanylate binding protein 5 | GBP5 | Guanylate-binding protein 5 | GBP-5
Origin Species
Human
SwissProt
Q96PP8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALEIHMSDP MCLIENFNEQ LKVNQEALEI LSAITQPVVV VAIVGLYRTG KSYLMNKLAG
61KNKGFSVAST VQSHTKGIWI WCVPHPNWPN HTLVLLDTEG LGDVEKADNK NDIQIFALAL
121LLSSTFVYNT VNKIDQGAID LLHNVTELTD LLKARNSPDL DRVEDPADSA SFFPDLVWTL
181RDFCLGLEID GQLVTPDEYL ENSLRPKQGS DQRVQNFNLP RLCIQKFFPK KKCFIFDLPA
241HQKKLAQLET LPDDELEPEF VQQVTEFCSY IFSHSMTKTL PGGIMVNGSR LKNLVLTYVN
301AISSGDLPCI ENAVLALAQR ENSAAVQKAI AHYDQQMGQK VQLPMETLQE LLDLHRTSER
361EAIEVFMKNS FKDVDQSFQK ELETLLDAKQ NDICKRNLEA SSDYCSALLK DIFGPLEEAV
421KQGIYSKPGG HNLFIQKTEE LKAKYYREPR KGIQAEEVLQ KYLKSKESVS HAILQTDQAL
481TETEKKKKEA QVKAEAEKAE AQRLAAIQRQ NEQMMQERER LHQEQVRQME IAKQNWLAEQ
541QKMQEQQMQE QAAQLSTTFQ AQNRSLLSEL QHAQRTVNND DPCVLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

586 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.