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GBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111772-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GBP3 (NM_018284), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTP-binding protein 3 | Guanylate binding protein 3 | GBP-3 | GBP3 | Guanylate-binding protein 3
Origin Species
Human
SwissProt
Q9H0R5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPEIHMTGP MCLIENTNGE LVANPEALKI LSAITQPVVV VAIVGLYRTG KSYLMNKLAG
61KNKGFSLGST VKSHTKGIWM WCVPHPKKPE HTLVLLDTEG LGDVKKGDNQ NDSWIFTLAV
121LLSSTLVYNS MGTINQQAMD QLYYVTELTH RIRSKSSPDE NENEDSADFV SFFPDFVWTL
181RDFSLDLEAD GQPLTPDEYL EYSLKLTQGT SQKDKNFNLP RLCIRKFFPK KKCFVFDLPI
241HRRKLAQLEK LQDEELDPEF VQQVADFCSY IFSNSKTKTL SGGIKVNGPR LESLVLTYIN
301AISRGDLPCM ENAVLALAQI ENSAAVQKAI AHYDQQMGQK VQLPAETLQE LLDLHRVSER
361EATEVYMKNS FKDVDHLFQK KLAAQLDKKR DDFCKQNQEA SSDRCSALLQ VIFSPLEEEV
421KAGIYSKPGG YCLFIQKLQD LEKKYYEEPR KGIQAEEILQ TYLKSKESVT DAILQTDQIL
481TEKEKEIEVE CVKAESAQAS AKMVEEMQIK YQQMMEEKEK SYQEHVKQLT EKMERERAQL
541LEEQEKTLTS KLQEQARVLK ERCQGESTQL QNEIQKLQKT LKKKTKRYMS HKLKI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

595 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.