Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GB5 / GNB5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115216-100
Catalog Number: LS-G115216
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GNB5 (NM_006578), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein | beta-5 subunit | Gbeta5 | beta subunit 5L | Transducin beta chain 5 | GB5 | GNB5
- Origin Species
- Human
- NCBI GenBank
- NM_006578
- SwissProt
- O14775
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATEGLHENE TLASLKSEAE SLKGKLEEER AKLHDVELHQ VAERVEALGQ FVMKTRRTLK61GHGNKVLCMD WCKDKRRIVS SSQDGKVIVW DSFTTNKEHA VTMPCTWVMA CAYAPSGCAI121ACGGLDNKCS VYPLTFDKNE NMAAKKKSVA MHTNYLSACS FTNSDMQILT ASGDGTCALW181DVESGQLLQS FHGHGADVLC LDLAPSETGN TFVSGGCDKK AMVWDMRSGQ CVQAFETHES241DINSVRYYPS GDAFASGSDD ATCRLYDLRA DREVAIYSKE SIIFGASSVD FSLSGRLLFA301GYNDYTINVW DVLKGSRVSI LFGHENRVST LRVSPDGTAF CSGSWDHTLR VWATRTRPLEQKLISEEDLAANDILDYKDDDDKV
353 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.