Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GAX / MEOX2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100680-100
Catalog Number: LS-G100680
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MEOX 2(MEOX2) (NM_005924), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MOX2 | MEOX2 | Mesenchyme homeobox 2 | GAX | Homeobox protein MOX-2
- Origin Species
- Human
- NCBI GenBank
- NM_005924
- SwissProt
- P50222
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEHPLFGCLR SPHATAQGLH PFSQSSLALH GRSDHMSYPE LSTSSSSCII AGYPNEEGMF61ASQHHRGHHH HHHHHHHHHQ QQQHQALQTN WHLPQMSSPP SAARHSLCLQ PDSGGPPELG121SSPPVLCSNS SSLGSSTPTG AACAPGDYGR QALSPAEAEK RSGGKRKSDS SDSQEGNYKS181EVNSKPRKER TAFTKEQIRE LEAEFAHHNY LTRLRRYEIA VNLDLTERQV KVWFQNRRMK241WKRVKGGQQG AAAREKELVN VKKGTLLPSE LSGIGAATLQ QTGDSIANED SHDSDHSSEH301AHLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
303 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.