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GATAD2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GATAD2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111074-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human p66 beta(GATAD2B) (NM_020699), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GATAD2B | KIAA1150 | p66beta | RP11-216N14.6 | p66/p68 | p68
Origin Species
Human
NCBI GenBank
NM_020699
SwissProt
Q8WXI9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRMTEDALR LNLLKRSLDP ADERDDVLAK RLKMEGHEAM ERLKMLALLK RKDLANLEVP
61HELPTKQDGS GVKGYEEKLN GNLRPHGDNR TAGRPGKENI NDEPVDMSAR RSEPERGRLT
121PSPDIIVLSD NEASSPRSSS RMEERLKAAN LEMFKGKGIE ERQQLIKQLR DELRLEEARL
181VLLKKLRQSQ LQKENVVQKT PVVQNAASIV QPSPAHVGQQ GLSKLPSRPG AQGVEPQNLR
241TLQGHSVIRS ATNTTLPHML MSQRVIAPNP AQLQGQRGPP KPGLVRTTTP NMNPAINYQP
301QSSSSVPCQR TTSSAIYMNL ASHIQPGTVN RVSSPLPSPS AMTDAANSQA AAKLALRKQL
361EKTLLEIPPP KPPAPLLHFL PSAANSEFIY MVGLEEVVQS VIDSQGKSCA SLLRVEPFVC
421AQCRTDFTPH WKQEKNGKIL CEQCMTSNQK KALKAEHTNR LKNAFVKALQ QEQEIEQRLQ
481QQAALSPTTA PAVSSVSKQE TIMRHHTLRQ APQPQSSLQR GIPTSARSML SNFAQAPQLS
541VPGGLLGMPG VNIAYLNTGI GGHKGPSLAD RQREYLLDMI PPRSISQSIS GQK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

593 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.