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GATAD2A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GATAD2A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112332-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human p66 alpha(GATAD2A) (NM_017660), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GATAD2A | Hp66alpha | p66 alpha | p66alpha
Origin Species
Human
NCBI GenBank
NM_017660
SwissProt
Q86YP4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.51 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIKQLKEELR LEEAKLVLLK KLRQSQIQKE ATAQKPTGSV GSTVTTPPPL VRGTQNIPAG
61KPSLQTSSAR MPGSVIPPPL VRGGQQASSK LGPQASSQVV MPPLVRGAQQ IHSIRQHSST
121GPPPLLLAPR ASVPSVQIQG QRIIQQGLIR VANVPNTSLL VNIPQPTPAS LKGTTATSAQ
181ANSTPTSVAS VVTSAESPAS RQAAAKLALR KQLEKTLLEI PPPKPPAPEM NFLPSAANNE
241FIYLVGLEEV VQNLLETQGR MSAATVLSRE PYMCAQCKTD FTCRWREEKS GAIMCENCMT
301TNQKKALKVE HTSRLKAAFV KALQQEQEIE QRLLQQGTAP AQAKAEPTAA PHPVLKQVIK
361PRRKLAFRSG EARDWSNGAV LQASSQLSRG SATTPRGVLH TFSPSPKLQN SASATALVSR
421TGRHSERTVS AGKGSATSNW KKTPLSTGGT LAFVSPSLAV HKSSSAVDRQ REYLLDMIPP
481RSIPQSATWK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

490 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.