Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GASP2 / GPRASP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107399-100
Catalog Number: LS-G107399
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPRASP2 (NM_138437), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GPRASP2 | GASP-2 | GASP2
- Origin Species
- Human
- SwissProt
- Q96D09
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTGAEIEPSA QAKPEKKAGE EVIAGPEREN DVPLVVRPKV RTQATTGARP KTETKSVPAA61RPKTEAQAMS GARPKTEVQV MGGARPKTEA QGITGARPKT DARAVGGARS KTDAKAIPGA121RPKDEAQAWA QSEFGTEAVS QAEGVSQTNA VAWPLATAES GSVTKSKGLS MDRELVNVDA181ETFPGTQGQK GIQPWFGPGE ETNMGSWCYS RPRAREEASN ESGFWSADET STASSFWTGE241ETSVRSWPRE ESNTRSRHRA KHQTNPRSRP RSKQEAYVDS WSGSEDEASN PFSFWVGENT301NNLFRPRVRE EANIRSKLRT NREDCFESES EDEFYKQSWV LPGEEANSRF RHRDKEDPNT361ALKLRAQKDV DSDRVKQEPR FEEEVIIGSW FWAEKEASLE GGASAICESE PGTEEGAIGG421SAYWAEEKSS LGAVAREEAK PESEEEAIFG SWFWDRDEAC FDLNPCPVYK VSDRFRDAAE481ELNASSRPQT WDEVTVEFKP GLFHGVGFRS TSPFGIPEEA SEMLEAKPKN LELSPEGEEQ541ESLLQPDQPS PEFTFQYDPS YRSVREIREH LRARESAESE SWSCSCIQCE LKIGSEEFEE601FLLLMDKIRD PFIHEISKIA MGMRSASQFT RDFIRDSGVV SLIETLLNYP SSRVRTSFLE661NMIHMAPPYP NLNMIETFIC QVCEETLAHS VDSLEQLTGI RMLRHLTMTI DYHTLIANYM721SGFLSLLTTA NARTKFHVLK MLLNLSENPA VAKKLFSAKA LSIFVGLFNI EETNDNIQIV781IKMFQNISNI IKSGKMSLID DDFSLEPLIS AFREFEELAK QLQAQIDNQN DPEVGQQSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
838 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.