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GAS2L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GAS2L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105084-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GAS2L3 (NM_174942), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
SwissProt
Q86XJ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQPAIQVWFG EDLPLSPRSP LTPRHGPGLA NVCQYDEWIA VRHEATLLPM QEDLSIWLSG
61LLGIKVKAEK LLEELDNGVL LCQLIDVLQN MVKTCNSEES GNFPMRKVPC KKDAASGSFF
121ARDNTANFLH WCRDIGVDET YLFESEGLVL HKDPRQVYLC LLEIGRIVSR YGVEPPVLVK
181LEKEIELEET LLNTSGPEDS ISIPKSCCRH EELHEAVKHI AEDPPCSCSH RFSIEYLSEG
241RYRLGDKILF IRMLHGKHVM VRVGGGWDTL QGFLLKYDPC RILQFATLEQ KILAFQKGVS
301NESVPDSPAR TPQPPEMNPL SAVNMFQKQN SKPSVPVSIP KSKEKQGRPP GALVPASSLK
361GGNLGSMSVR SKLPNSPAAS SHPKLKSSKG ITKKPQAPSN NASSSLASLN PVGKNTSSPA
421LPRTAPCISE SPRKCISSPN TPKAKVIPAQ NSADLPESTL LPNKCSGKTQ PKYLKHNHIS
481SRDNAVSHLA AHSNSSSKCP KLPKANIPVR PKPSFQSSAK MTKTSSKTIA TGLGTQSQPS
541DGAPQAKPVP AQKLKSALNL NQPVSVSSVS PVKATQKSKD KNIVSATKKQ PQNKSAFQKT
601GPSSLKSPGR TPLSIVSLPQ SSTKTQTAPK SAQTVAKSQH STKGPPRSGK TPASIRKPPS
661SVKDADSGDK KPTAKKKEDD DHYFVMTGSK KPRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

694 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.