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GAS2L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GAS2L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115283-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GAS2L1 (NM_006478), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GAS2-like protein 1 | GAS2L1 | GAR22
Origin Species
Human
NCBI GenBank
NM_006478
SwissProt
Q99501

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADPVAGIAG SAAKSVRPFR SSEAYVEAMK EDLAEWLNAL YGLGLPGGGD GFLTGLATGT
61TLCQHANAVT EAARALAAAR PARGVAFQAH SVVPGSFMAR DNVATFIGWC RVELGVPEVL
121MFETEDLVLR KNEKSVVLCL LEVARRGARL GLLAPRLVQF EQEIERELRA APPAPNAPAA
181GEDTTETAPA PGTPARGPRM TPSDLRNLDE LVREILGRCT CPDQFPMIKV SEGKYRVGDS
241SLLIFVRVLR SHVMVRVGGG WDTLEHYLDK HDPCRCSSTA HRPPQPRVCT FSPQRVSPTT
301SPRPASPVPG SERRGSRPEM TPVSLRSTKE GPETPPRPRD QLPPHPRSRR YSGDSDSSAS
361SAQSGPLGTR SDDTGTGPRR ERPSRRLTTG TPASPRRPPA LRSQSRDRLD RGRPRGAPGG
421RGAQLSVPSP ARRARSQSRE EQAVLLVRRD RDGQHSWVPR GRGSGGSGRS TPQTPRARSP
481AAPRLSRVSS PSPELGTTPA SIFRTPLQLD PQQEQQLFRR LEEEFLANAR ALEAVASVTP
541TGPAPDPARA PDPPAPDSAY CSSSSSSSSL SVLGGKCGQP GDSGRTANGL PGPRSQALSS
601SSDEGSPCPG MGGPLDAPGS PLACTEPSRT WARGRMDTQP DRKPSRIPTP RGPRRPSGPA
661ELGTWHALHS VTPRAEPDSW M
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

681 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.