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GARNL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GARNL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108930-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GARNL3 (NM_032293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA356B19.1 | GARNL3 | RP11-356B19.1
Origin Species
Human
NCBI GenBank
NM_032293
SwissProt
Q5VVW2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
110.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKHFCSSSVS EDLGCRRGDF SRKHYGSVEL LISSDADGAI QRAGRFRVEN GSSDENATAL
61PGTWRRTDVH LENPEYHTRW YFKYFLGQVR QNYIGNDAEK SPFFLSVTLS DQNNQRVPQY
121RAILWRKTGT QKICLPYSPT KTLSVKSILS AMNLDKFEKG PREIFHPEIQ KDLLVLEEQE
181GSVNFKFGVL FAKDGQLTDD EMFSNEIGSE PFQKFLNLLG DTITLKGWTG YRGGLDTKND
241TTGIHSVYTV YQGHEIMFHV STMLPYSKEN KQQVERKRHI GNDIVTIVFQ EGEESSPAFK
301PSMIRSHFTH IFALVRYNQQ NDNYRLKIFS EESVPLFGPP LPTPPVFTDH QEFRDFLLVK
361LINGEKATLE TPTFAQKRRR TLDMLIRSLH QDLMPDLHKN MLNRRSFSDV LPESPKSARK
421KEEARQAEFV RIGQALKLKS IVRGDAPSSL AASGICKKEP WEPQCFCSNF PHEAVCADPW
481GQALLVSTDA GVLLVDDDLP SVPVFDRTLP VKQMHVLETL DLLVLRADKG KDARLFVFRL
541SALQKGLEGK QAGKSRSDCR ENKLEKTKGC HLYAINTHHS RELRIVVAIR NKLLLITRKH
601NKPSGVTSTS LLSPLSESPV EEFQYIREIC LSDSPMVMTL VDGPAEESDN LICVAYRHQF
661DVVNESTGEA FRLHHVEANR VNFVAAIDVY EDGEAGLLLC YNYSCIYKKV CPFNGGSFLV
721QPSASDFQFC WNQAPYAIVC AFPYLLAFTT DSMEIRLVVN GNLVHTAVVP QLQLVASRSD
781IYFTATAAVN EVSSGGSSKG ASARNSPQTP PGRDTPVFPS SLGEGEIQSK NLYKIPLRNL
841VGRSIERPLK SPLVSKVITP PTPISVGLAA IPVTHSLSLS RMEIKEIASR TRRELLGLSD
901EGGPKSEGAP KAKSKPRKRL EESQGGPKPG AVRSSSSDRI PSGSLESAST SEANPEGHSA
961SSDQDPVADR EGSPVSGSSP FQLTAFSDED IIDLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

995 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.