Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GAPIII / RASA3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114681-100
Catalog Number: LS-G114681
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASA3 (NM_007368), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GAPIII | GAP1(IP4BP) | GTPase activating protein III | Ins(1 | 3 | 4 | 5)P4-binding protein | GAP1IP4BP | Ins P4-binding protein | RAS p21 protein activator 3 | RASA3
- Origin Species
- Human
- NCBI GenBank
- NM_007368
- SwissProt
- Q14644
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAVEDEGLRV FQSVKIKIGE AKNLPSYPGP SKMRDCYCTV NLDQEEVFRT KIVEKSLCPF61YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE DLQKYHNRDT WFQLQHVDAD121SEVQGKVHLE LRLSEVITDT GVVCHKLATR IVECQGLPIV NGQCDPYATV TLAGPFRSEA181KKTKVKRKTN NPQFDEVFYF EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD241EFLGELRIPL KVLRQSSSYE AWYFLQPRDN GSKSLKPDDL GSLRLNVVYT EDHVFSSDYY301SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAVPLVR LFLHYGRVVP FISAIASAEV361KRTQDPNTIF RGNSLASKCI DETMKLAGMH YLHVTLKPAI EEICQSHKPC EIDPVKLKDG421ENLENNMENL RQYVDRVFHA ITESGVSCPT VMCDIFFSLR EAAAKRFQDD PDVRYTAVSS481FIFLRFFAPA ILSPNLFQLT PHHTDPQTSR TLTLISKTVQ TLGSLSKSKS ASFKESYMAT541FYEFFNEQKY ADAVKNFLDL ISSSGRRDPK SVEQPIVLKE GFMIKRAQGR KRFGMKNFKK601RWFRLTNHEF TYHKSKGDQP LYSIPIENIL AVEKLEEESF KMKNMFQVIQ PERALYIQAN661NCVEAKDWID ILTKVSQCNQ KRLTVYHPSA YLSGHWLCCR APSDSAPGCS PCTGGLPANI721QLDIDGDRET ERIYSLFNLY MSKLEKMQEA CGSKSVYDGP EQEEYSTFVI DDPQETYKTL781KQVIAGVGAL EQEHAQYKRD KFKKTKYGSQ EHPIGDKSFQ NYIRQQSETS THSITRTRPLEQKLISEEDLAANDILDYKDDDDKV
834 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.