Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GALR1 / Galanin Receptor 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118573-100
Catalog Number: LS-G118573
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GALR1 (NM_001480), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Galanin receptor type 1 | GALR1 | Galanin receptor 1 | Galanin receptor galr1 | Galanin-1 receptor | GALNR1 | Gal receptor 1 | Gal-r1 | Galn1r | Gal-1 receptor | GAL1-R | Galanin r1 receptor | GALNR | GALR-1 | Hugal 1-r
- Origin Species
- Human
- NCBI GenBank
- NM_001480
- SwissProt
- P47211
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MELAVGNLSE GNASWPEPPA PEPGPLFGIG VENFVTLVVF GLIFALGVLG NSLVITVLAR61SKPGKPRSTT NLFILNLSIA DLAYLLFCIP FQATVYALPT WVLGAFICKF IHYFFTVSML121VSIFTLAAMS VDRYVAIVHS RRSSSLRVSR NALLGVGCIW ALSIAMASPV AYHQGLFHPR181ASNQTFCWEQ WPDPRHKKAY VVCTFVFGYL LPLLLICFCY AKVLNHLHKK LKNMSKKSEA241SKKKTAQTVL VVVVVFGISW LPHHIIHLWA EFGVFPLTPA SFLFRITAHC LAYSNSSVNP301IIYAFLSENF RKAYKQVFKC HIRKDSHLSD TKENKSRIDT PPSTNCTHVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
349 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.