Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GALNT16 / GALNTL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111067-100
Catalog Number: LS-G111067
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GALNTL1(GALNT16) (NM_020692), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GALNTL1 | GALNACT16 | Pp-GaNTase-like protein 1 | KIAA1130 | GalNAc-T-like protein 1 | GALNT16
- Origin Species
- Human
- NCBI GenBank
- AB032956
- SwissProt
- Q8N428
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRKIRANAIA ILTVAWILGT FYYLWQDNRA HAASSGGRGA QRAGRRSEQL REDRTIPLIV61TGTPSKGFDE KAYLSAKQLK AGEDPYRQHA FNQLESDKLS PDRPIRDTRH YSCPSVSYSS121DLPATSVIIT FHNEARSTLL RTVKSVLNRT PANLIQEIIL VDDFSSDPED CLLLTRIPKV181KCLRNDRREG LIRSRVRGAD MAAATVLTFL DSHCEVNTEW LPPMLQRVKE DHTRVVSPII241DVISLDNFAY LAASADLRGG FDWSLHFKWE QIPLEQKMTR TDPTRPIRTP VIAGGIFVID301KSWFNHLGKY DAQMDIWGGE NFELSFRVWM CGGSLEIVPC SRVGHVFRKR HPYNFPEGNA361LTYIRNTKRT AEVWMDEYKQ YYYEARPSAI GKAFGSVATR IEQRKKMNCK SFRWYLENVY421PELTVPVKEA LPGIIKQGVN CLESQGQNTA GDFLLGMGIC RGSAKNPQPA QAWLFSDHLI481QQQGKCLAAT STLMSSPGSP VILQMCNPRE GKQKWRRKGS FIQHSVSGLC LETKPAQLVT541SKCQADAQAQ QWQLLPHTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
558 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.