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GALNT10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GALNT10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103785-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GALNT10 (NM_198321), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GalNAc transferase 10 | GALNT10 | GalNac-T10 | GALNACT10 | PPGALNACT10 | PPGANTASE10 | Pp-GaNTase 10
Origin Species
Human
NCBI GenBank
NM_017540
SwissProt
Q86SR1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRKEKRLLQ AVALVLAALV LLPNVGLWAL YRERQPDGTP GGSGAAVAPA AGQGSHSRQK
61KTFFLGDGQK LKDWHDKEAI RRDAQRVGNG EQGRPYPMTD AERVDQAYRE NGFNIYVSDK
121ISLNRSLPDI RHPNCNSKRY LETLPNTSII IPFHNEGWSS LLRTVHSVLN RSPPELVAEI
181VLVDDFSDRE HLKKPLEDYM ALFPSVRILR TKKREGLIRT RMLGASVATG DVITFLDSHC
241EANVNWLPPL LDRIARNRKT IVCPMIDVID HDDFRYETQA GDAMRGAFDW EMYYKRIPIP
301PELQKADPSD PFESPVMAGG LFAVDRKWFW ELGGYDPGLE IWGGEQYEIS FKVWMCGGRM
361EDIPCSRVGH IYRKYVPYKV PAGVSLARNL KRVAEVWMDE YAEYIYQRRP EYRHLSAGDV
421AVQKKLRSSL NCKSFKWFMT KIAWDLPKFY PPVEPPAAAW GEIRNVGTGL CADTKHGALG
481SPLRLEGCVR GRGEAAWNNM QVFTFTWRED IRPGDPQHTK KFCFDAISHT SPVTLYDCHS
541MKGNQLWKYR KDKTLYHPVS GSCMDCSESD HRIFMNTCNP SSLTQQWLFE HTNSTVLEKF
601NRN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

603 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.