Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GALNAC4ST1 / CHST8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110349-100
Catalog Number: LS-G110349
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHST8 (NM_022467), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CHST8 | GALNAC-4-ST1 | GalNAc4ST | GalNAc4ST-1 | GALNAC4ST1 | GalNAc-4-O-sulfotransferase 1
- Origin Species
- Human
- NCBI GenBank
- NM_022467
- SwissProt
- Q9H2A9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTLRPGTMRL ACMFSSILLF GAAGLLLFIS LQDPTELAPQ QVPGIKFNIR PRQPHHDLPP61GGSQDGDLKE PTERVTRDLS SGAPRGRNLP APDQPQPPLQ RGTRLRLRQR RRRLLIKKMP121AAATIPANSS DAPFIRPGPG TLDGRWVSLH RSQQERKRVM QEACAKYRAS SSRRAVTPRH181VSRIFVEDRH RVLYCEVPKA GCSNWKRVLM VLAGLASSTA DIQHNTVHYG SALKRLDTFD241RQGILHRLST YTKMLFVREP FERLVSAFRD KFEHPNSYYH PVFGKAILAR YRANASREAL301RTGSGVRFPE FVQYLLDVHR PVGMDIHWDH VSRLCSPCLI DYDFVGKFES MEDDANFFLS361LIRAPRNLTF PRFKDRHSQE ARTTARIAHQ YFAQLSALQR QRTYDFYYMD YLMFNYSKPF421ADLYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
424 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.