Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GALNAC4ST-2 / CHST9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109228-100
Catalog Number: LS-G109228
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHST9 (NM_031422), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GalNAc-4-sulfotransferase 2 | GalNAc-4-O-sulfotransferase 2 | GALNAC4ST-2 | CHST9 | GalNAc-4-ST2
- Origin Species
- Human
- NCBI GenBank
- NM_031422
- SwissProt
- Q7L1S5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVMNPKQVFL SVLIFGVAGL LLFMYLQVWI EEQHTGRVEK RREQKVTSGW GPVKYLRPVP61RIMSTEKIQE HITNQNPKFH MPEDVREKKE NLLLNSERST RLLTKTSHSQ GGDQALSKST121GSPTEKLIEK RQGAKTVFNK FSNMNWPVDI HPLNKSLVKD NKWKKTEETQ EKRRSFLQEF181CKKYGGVSHH QSHLFHTVSR IYVEDKHKIL YCEVPKAGCS NWKRILMVLN GLASSAYNIS241HNAVHYGKHL KKLDSFDLKG IYTRLNTYTK AVFVRDPMER LVSAFRDKFE HPNSYYHPVF301GKAIIKKYRP NACEEALING SGVKFKEFIH YLLDSHRPVG MDIHWEKVSK LCYPCLINYD361FVGKFETLEE DANYFLQMIG APKELKFPNF KDRHSSDERT NAQVVRQYLK DLTRTERQLI421YDFYYLDYLM FNYTTPFLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
438 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.