Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GABRQ / THETA Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101579-100
Catalog Number: LS-G101579
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GABRQ (NM_018558), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GABA(A) receptor subunit theta | GABA-A receptor theta subunit | THETA | GABA(A) receptor | theta | GABRQ
- Origin Species
- Human
- NCBI GenBank
- NM_018558
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGIRGMLRAA VILLLIRTWL AEGNYPSPIP KFHFEFSSAV PEVVLNLFNC KNCANEAVVQ61KILDRVLSRY DVRLRPNFGG APVPVRISIY VTSIEQISEM NMDYTITMFF HQTWKDSRLA121YYETTLNLTL DYRMHEKLWV PDCYFLNSKD AFVHDVTVEN RVFQLHPDGT VRYGIRLTTT181AACSLDLHKF PMDKQACNLV VESYGYTVED IILFWDDNGN AIHMTEELHI PQFTFLGRTI241TSKEVYFYTG SYIRLILKFQ VQREVNSYLV QVYWPTVLTT ITSWISFWMN YDSSAARVTI301GLTSMLILTT IDSHLRDKLP NISCIKAIDI YILVCLFFVF LSLLEYVYIN YLFYSRGPRR361QPRRHRRPRR VIARYRYQQV VVGNVQDGLI NVEDGVSSLP ITPAQAPLAS PESLGSLTST421SEQAQLATSE SLSPLTSLSG QAPLATGESL SDLPSTSEQA RHSYGVRFNG FQADDSIIPT481EIRNRVEAHG HGVTHDHEDS NESLSSDERH GHGPSGKPML HHGEKGVQEA GWDLDDNNDK541SDCLAIKEQF KCDTNSTWGL NDDELMAHGQ EKDSSSESED SCPPSPGCSF TEGFSFDLFN601PDYVPKVDKW SRFLFPLAFG LFNIVYWVYH MYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
632 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.